# Plaque 2.0
###a virological assay reloaded - fork us on [GitHub](https://github.com/plaque2/matlab)
![Plaque 2.0](img/LOGO.png "Plaque 2.0")
###[Home](index.html) : [Download](download.html) : [Help](help.html)
## How to cite us:
Yakimovich, A., Andriasyan, V., Witte, R., Wang, I. H., Prasad, V., Suomalainen, M., & Greber, U. F. (2015). Plaque2. 0—A High-Throughput Analysis Framework to Score Virus-Cell Transmission and Clonal Cell Expansion. PloS one, 10(9), [e0138760](http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138760).
Developed in 1950s, plaque assay started the era of the quantitative virology, allowing precise tittering and subsequent purification of infectious units from inoculae of various etiologies. However more than a means to titer viruses, *plaque assay* delivers phenotypes bearing information about the life cycle and spreading mechanism of viruses. Recent advance in automated high-throughput imaging and sCMOS camera technologies together with state-of-the-art image analysis algorithms can help harnessing this information. Here we present **Plaque2.0** – an assay framework bridging high-throughput high resolution midrange magnification imaging accompanied by image analysis software aimed at enabling researchers to maximize information they obtain from their plaque assays.
## Short demo of the Plaque2.0 Software installation and usage:
## Current Release: 2.0141118
The most recent version of the software is available in dev branch on GitHub for forking, cloning and download under GPLv3 open source license from [github.com/plaque2](http://github.com/plaque2).